产品名称
DICER Rabbit Polyclonal Antibody
基因名称
DICER1 DICER HERNA KIAA0928
蛋白名称
Endoribonuclease Dicer (EC 3.1.26.-) (Helicase with RNase motif) (Helicase MOI)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9UPY3
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9UPY3/entry
Mouse Swissprot No.
Q8R418
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8R418
免疫原
Synthesized peptide derived from part region of human protein
特异性
DICER Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010],
细胞定位
Cytoplasm . Cytoplasm, perinuclear region .
功能
cofactor:Binds 2 magnesium or manganese ions per subunit.,function:Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.,online information:The dark side of RNA -Issue 87 of October 2007,similarity:Belongs to the helicase family. Dicer subfamily.,similarity:Contains 1 Dicer dsRNA-binding fold domain.,similarity:Contains 1 DRBM (double-stranded RNA-binding) domain.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 1 PAZ domain.,similarity:Contains 2 RNase III domains.,subunit:Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, EIF2C1, PIWIL1 and PRKRA. Associates with the 60S ribosome.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
