产品名称
NUCL Rabbit Polyclonal Antibody
蛋白名称
Nucleolin (Protein C23)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P19338
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P19338/entry
Mouse Swissprot No.
P09405
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P09405
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P13383
免疫原
Synthesized peptide derived from part region of human protein
特异性
NUCL Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008],
组织表达
Brain,Cervix carcinoma,Epithelium,Hair follicle dermal papilla,Muscle,Retina,
细胞定位
Nucleus, nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
信号通路
Pathogenic Escherichia coli infection;
功能
function:Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.,sequence caution:Unlikely isoform. Aberrant splice sites.,similarity:Contains 4 RRM (RNA recognition motif) domains.,subunit:Interacts with APTX and NSUN2. Component of the SWAP complex that consists of NPM1, NCL/nucleolin, PARP1 and SWAP70. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
