产品名称
CD8B Rabbit Polyclonal Antibody
蛋白名称
T-cell surface glycoprotein CD8 beta chain (CD antigen CD8b)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P10966
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P10966/entry
Mouse Swissprot No.
P10300
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P10300
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P05541
免疫原
Synthesized peptide derived from part region of human protein
特异性
CD8B Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010],
组织表达
Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
细胞定位
[Isoform 1]: Cell membrane ; Single-pass type I membrane protein . Requires the partner CD8A for efficient cell surface expression (PubMed:3145196). The heterodimer CD8A/CD8B localizes to lipid rafts due to CD8B cytoplasmic tail palmitoylation. .; [Isoform 2]: Cell membrane ; Single-pass type I membrane protein .; [Isoform 3]: Secreted .; [Isoform 4]: Cell membrane ; Single-pass type I membrane protein .; [Isoform 5]: Cell membrane ; Single-pass type I membrane protein .; [Isoform 6]: Secreted .; [Isoform 7]: Secreted .; [Isoform 8]: Secreted .
信号通路
Cell adhesion molecules (CAMs);Antigen processing and presentation;Hematopoietic cell lineage;T_Cell_Receptor;Primary immunodeficiency;
功能
function:Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.,online information:CD8 entry,PTM:Phosphorylated as a consequence of T-cell activation .,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:In general heterodimer of an alpha and a beta chain linked by two disulfide bonds.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
