产品名称
LIMA1 Rabbit Polyclonal Antibody
基因名称
LIMA1 EPLIN SREBP3 PP624
蛋白名称
LIM domain and actin-binding protein 1 (Epithelial protein lost in neoplasm)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9UHB6
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9UHB6/entry
Mouse Swissprot No.
Q9ERG0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9ERG0
免疫原
Synthesized peptide derived from part region of human protein AA range: 631-681
特异性
LIMA1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011],
组织表达
Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium.
细胞定位
Cytoplasm. Cell junction, focal adhesion . Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber . Cell membrane . Expressed in the brush border membrane of the small intestine and colocalizes with NPC1L1 and MYO5B (PubMed:29880681). Colocalizes with PXN at focal adhesions in mesangial cells (PubMed:24694988). Colocalizes with actin stress fibers in quiescent cells. PDGF stimulation induced disassembly of stress fibers and formation of peripheral and dorsal ruffles, where LIMA1 is relocalized (By similarity). .
功能
domain:Contains at least 2 actin-binding domains, one on each side of the LIM domain. Both domains bind actin monomers and filaments. The C-terminal domain binds filaments more efficiently than the N-terminus.,function:Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.,induction:Down-regulated in some cancer cell lines. Isoform Alpha induced by serum. Isoform Beta constitutively expressed.,similarity:Contains 1 LIM zinc-binding domain.,subcellular location:This cytoskeletal protein co-localizes with actin stress fibers and focal adhesion plaques.,tissue specificity:Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
