产品名称
H2AZ Rabbit Polyclonal Antibody
蛋白名称
Histone H2A.Z (H2A/z)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P0C0S5
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P0C0S5/entry
Mouse Swissprot No.
P0C0S6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P0C0S6
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P0C0S7
免疫原
Synthesized peptide derived from part region of human protein AA range: 1-80
特异性
H2AZ Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008],
组织表达
Brain,Epithelium,Skeletal muscle,Uterus,
细胞定位
Nucleus. Chromosome.
信号通路
Systemic lupus erythematosus;
功能
function:Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.,mass spectrometry:Monoisotopic, not modified PubMed:16457589,PTM:Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.,PTM:Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.,PTM:Not phosphorylated.,similarity:Belongs to the histone H2A family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
