产品名称
DOCK4 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/9732
Human Swissprot No.
Q8N1I0
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q8N1I0/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/238130
Mouse Swissprot No.
P59764
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P59764
免疫原
Synthesized peptide derived from human DOCK4 AA range: 533-583
特异性
This antibody detects endogenous levels of DOCK4 at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated, cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described, but only one has been fully characterized. [provided by RefSeq, Jul 2008],
组织表达
Widely expressed at low level. Highly expressed in skeletal muscle, prostate and ovary. ; [Isoform 2]: May be specifically expressed in the brain and eye.
细胞定位
Cell membrane . Cell projection . Cytoplasm, cytosol . Colocalizes with EPHA2, RHOG and CTTN/cortactin at the tip of protrusions in migrating cells. .
功能
disease:Defects in DOCK4 are involved in the cause of some cancers, which are probably due to lack of Rap1 activation.,domain:The DHR-2 domain probably mediates the GEF activity.,function:Involved in regulation of adherens junction between cells. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP.,miscellaneous:Injection of wild-type protein suppresses tumor invasion in osteosarcoma mouse cell lines while it is not the case with the Leu-1718 variant.,similarity:Belongs to the DOCK family.,similarity:Contains 1 DHR-1 (CZH-1) domain.,similarity:Contains 1 DHR-2 (CZH-2) domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with the SH3 domain of CRK. Interacts with nucleotide-free Rap1.,tissue specificity:Widely expressed at low level. Highly expressed in skeletal muscle, prostate and ovary.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
