产品名称
UBC12 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/9040
Human Swissprot No.
P61081
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P61081/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/22192
Mouse Swissprot No.
P61082
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P61082
免疫原
Synthesized peptide derived from human UBC12 AA range: 37-87
特异性
This antibody detects endogenous levels of UBC12 at Human/Mouse
宿主
Polyclonal, Rabbit,IgG
背景介绍
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008],
细胞定位
cytoplasm,cytosol,extracellular exosome,
功能
catalytic activity:ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.,domain:Both the N-terminal docking peptide and the catalytic core domain must bind the uba3-nae1 complex simultaneously for optimal transfer of nedd8.,function:Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase rbx1, but not rbx2, suggests that the rbx1-ube2m complex neddylates specific target proteins, such as cul1, cul2, cul3 and cul4. Involved in cell proliferation.,pathway:Protein modification; protein neddylation.,similarity:Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.,subunit:Interacts with UBA3 and RBX1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
