产品名称
IRGM Rabbit Polyclonal Antibody
基因名称
IRGM IFI1 IRGM1 LRG47
蛋白名称
Immunity-related GTPase family M protein (EC 3.6.5.-) (Immunity-related GTPase family M protein 1) (Interferon-inducible protein 1) (LPS-stimulated RAW 264.7 macrophage protein 47 homolog) (LRG-47)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
A1A4Y4
Human Swissprot Link
https://www.uniprot.org/uniprotkb/A1A4Y4/entry
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q6AYC2
免疫原
Synthesized peptide derived from human protein . at AA range: 101-150
特异性
IRGM Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
immunity related GTPase M(IRGM) Homo sapiens This gene encodes a member of the p47 immunity-related GTPase family. The encoded protein may play a role in the innate immune response by regulating autophagy formation in response to intracellular pathogens. Polymorphisms that affect the normal expression of this gene are associated with a susceptibility to Crohn's disease and tuberculosis.[provided by RefSeq, Oct 2010],
组织表达
Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes.
细胞定位
Golgi apparatus membrane . Cell membrane . Cytoplasmic vesicle, phagosome membrane . Cytoplasmic vesicle, autophagosome membrane . Cell projection, phagocytic cup . Behaves like an integral membrane protein. Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes. Preferentially associated with cis- and medial-Golgi. .
功能
disease:Defects in IRGM are associated with susceptibility to Crohn disease (CD) [MIM:266600]. CD is a form of remitting inflammatory bowel disease (IBD). CD may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous. CD is commonly classified as an autoimmune disease. The causal variants do not affect the protein sequence but may lie in regulatory regions and alter its expression.,function:Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility.,induction:Not up-regulated by IFNG.,miscellaneous:Depletion of IRGM by siRNA favors mycobacteria survival in macrophages.,miscellaneous:There is a huge difference in terms of sequence and regulation of expression compared to the mouse ortholog and hence, the function might be slightly different (PubMed:16277747).,similarity:Belongs to the interferon-inducible GTPase family.,subcellular location:Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi.,tissue specificity:Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
