产品名称
JPH2 Rabbit Polyclonal Antibody
蛋白名称
Junctophilin-2 (JP-2) (Junctophilin type 2)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9BR39
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9BR39/entry
Mouse Swissprot No.
Q9ET78
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9ET78
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q2PS20
免疫原
Synthesized peptide derived from human protein . at AA range: 611-660
特异性
JPH2 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. This gene is a member of the junctophilin gene family. Alternative splicing has been observed at this locus and two variants encoding distinct isoforms are described. [provided by RefSeq, Jul 2008],
组织表达
Specifically expressed in skeletal muscle and heart.
细胞定位
[Junctophilin-2]: Cell membrane ; Peripheral membrane protein . Sarcoplasmic reticulum membrane ; Single-pass type IV membrane protein . Endoplasmic reticulum membrane ; Single-pass type IV membrane protein . The transmembrane domain is anchored in sarcoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane. In heart cells, it predominantly associates along Z lines within myocytes. In skeletal muscle, it is specifically localized at the junction of A and I bands. .; [Junctophilin-2 N-terminal fragment]: Nucleus . Accumulates in the nucleus of stressed hearts. .
功能
domain:The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids.,function:Contributes to the stabilization of the junctional membrane complexes, which are common to excitable cells and mediate cross-talk between cell surface and intracellular ion channels. Probably acts by anchoring the plasma membrane and endoplasmic/sarcoplasmic reticulum. Contributes to the construction of skeletal muscle triad junctions, and plays an essential role in heart development.,similarity:Belongs to the junctophilin family.,similarity:Contains 8 MORN repeats.,subcellular location:Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic/sarcoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane. In heart cells, it predominantly associates along Z lines within myocytes. In skeletal muscle, it is specifically localized at the junction of A and I bands.,tissue specificity:Specifically expressed in skeletal muscle and heart.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
