产品名称
SPRE1 Rabbit Polyclonal Antibody
蛋白名称
Sprouty-related, EVH1 domain-containing protein 1 (Spred-1) (hSpred1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q7Z699
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q7Z699/entry
Mouse Swissprot No.
Q924S8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q924S8
免疫原
Synthesized peptide derived from human protein . at AA range: 210-290
特异性
SPRE1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq, Jul 2008],
组织表达
Weakly expressed in embryonic cell line HEK293.
细胞定位
Cell membrane ; Peripheral membrane protein . Membrane, caveola ; Peripheral membrane protein . Nucleus . Localized in cholesterol-rich membrane raft/caveola fractions.
功能
disease:Defects in SPRED1 are the cause of neurofibromatosis type 1-like syndrome (NFLS) [MIM:611431]. Neurofibromatosis type 1 (NF1) is one of the most frequent autosomal dominant diseases. It belongs to the group of disorders known as the 'neuro-cardio-facial-cutaneous' syndromes, present with a variable degree of cognitive impairment, facial dysmorphism, congenital heart defects and skin abnormalities. NFLS is a form of these disorders with autosomal dominant trait consisting of multiple cafe-au-lait spots, axillary freckling, macrocephaly and a Noonan-like dysmorphy in some individuals.,function:Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow.,PTM:Phosphorylated on tyrosine.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 1 KBD domain.,similarity:Contains 1 SPR (sprouty) domain.,similarity:Contains 1 WH1 domain.,subcellular location:Localized in cholesterol-rich membrane raft/caveola fractions.,subunit:Interacts with Ras (By similarity). Homodimer and heterodimer. Interacts with CAV1. Able to interact with SPRED2 to form heterodimers.,tissue specificity:Weakly expressed in embryonic cell line (HEK-293).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
