产品名称
HECAM Rabbit Polyclonal Antibody
蛋白名称
Hepatocyte cell adhesion molecule (Protein hepaCAM)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q14CZ8
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q14CZ8/entry
Mouse Swissprot No.
Q640R3
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q640R3
免疫原
Synthesized peptide derived from human protein . at AA range: 10-90
特异性
HECAM Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a single-pass type I membrane protein that localizes to the cytoplasmic side of the cell membrane. The encoded protein acts as a homodimer and is involved in cell motility and cell-matrix interactions. The expression of this gene is downregulated or undetectable in many cancer cell lines, so this may be a tumor suppressor gene. [provided by RefSeq, Jul 2011],
组织表达
Brain,Fetal brain,Hypothalamus,Liver,
细胞定位
Cytoplasm . Membrane; Single-pass type I membrane protein; Cytoplasmic side . In MCF-7 breast carcinoma and hepatic Hep 3B2.1-7 and Hep-G2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.
功能
caution:Product of a dubious CDS prediction. Encoded by the 3'-UTR of HEPACAM.,domain:The cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility.,function:Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.,induction:Down-regulated in 20 out of 23 of hepatocellular carcinoma (HCC) samples and is undetectable in 5 HCC cell lines tested.,PTM:N-glycosylated.,similarity:Contains 1 Ig-like C2-type (immunoglobulin-like) domain.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subcellular location:In MCF7 breast carcinoma and hepatic Hep3B and HepG2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.,subunit:Homodimer. Dimer formation occurs predominantly through cis interactions on the cell surface.,tissue specificity:Expressed in liver. Expression is either downregulated or lost in hepatocellular carcinomas (HCC).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
