BD-PE5720-STAT3 (11J12) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

STAT3 (11J12) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5720

产品名称

STAT3 (11J12) Mouse Monoclonal antibody

类别

常规抗体

基因名称

STAT3

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Recombinant protein expressed in E.coli corresponding to amino acids 154-175 of human stat3

稀释度

WB: 1:500, IHC 1:50

预测分子量

88.08kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a member of the STAT protein family. In response to cytokinesand growth factors, STAT family members are phosphorylated by the receptor associated kinases, andthen form homo- or heterodimers that translocate to the cell nucleus where they act astranscription activators. This protein is activated through phosphorylation in response to variouscytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This proteinmediates the expression of a variety of genes in response to cell stimuli, and thus plays a keyrole in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has beenshown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor ofthis protein. Three alternatively spliced transcript variants encoding distinct isoforms have beendescribed

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-STAT3 (BD-PE5720) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-STAT3 (BD-PE5720) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

Immunohistochemical staining of paraffin-embedded thyroid within the normal limits using anti-STAT3 (BD-PE5720) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

Immunohistochemical staining of paraffin-embedded colon within the normal limits using anti-STAT3 (BD-PE5720) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-STAT3 (BD-PE5720) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-STAT3 (BD-PE5720) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-STAT3 (BD-PE5720) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

Western blot analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-STAT3 (BD-PE5720) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3(Cat# BD-PE5720). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.