Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PRKAR1A monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded normal breast using anti-PRKAR1A C/N BD-PE5713 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded breast cancer using anti-PRKAR1A C/N BD-PE5713 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5713, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded renal cancer using anti-PRKAR1A C/N BD-PE5713 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR1A monoclonal antibody.

Immunohistochemical staining of paraffin-embedded colon cancer using anti-PRKAR1A C/N BD-PE5713 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5713, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded normal colon using anti-PRKAR1A C/N BD-PE5713 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded melanoma using anti-PRKAR1A C/N BD-PE5713 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, anti-PRKAR1A diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5713, Dilution 1:50)

Figure from citation: PRKAR1A IHC in a CNC-associated cardiac myxoma. A, A photomicrograph of the specimen at low power exhibits the robust reactivity of the adjacent normal myocardium with antibodies directed against PRKAR1A. B, A high-power photomicrograph exhibits the lack of reactivity seen in the neoplastic (myxoma) cells, although the intratumoral histiocytes are still strongly reactive. Dilution: 1:8000

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5713, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5713, Dilution 1:50)

Anti-PRKAR1A mouse monoclonal antibody (BD-PE5713) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1A .

Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5713, Dilution 1:50)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A (Cat# BD-PE5713). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.