BD-PE5695-KEAP1 (14X1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

KEAP1 (14X1) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5695

产品名称

KEAP1 (14X1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

KEAP1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human KEAP1 (NP_987096) produced in HEK293T cell.

稀释度

WB 1:2000, IF 1:100, FCM 1:100

预测分子量

69.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody (BD-PE5695), and then analyzed by flow cytometry.

Anti-KEAP1 mouse monoclonal antibody (BD-PE5695) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KEAP1 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).