Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-APOBEC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE5694) (1:500).

Western blot analysis of extracts (35ug) from HT29 cell line and mouse testis tissue lysate by using anti-APOBEC4 monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APOBEC4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APOBEC4 (Cat# BD-PE5694)(1:2000).