Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CLU monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE5684) (1:150)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE5684) (1:150)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE5684) (1:150)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE5684) (1:150)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE5684) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CLU mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE5684) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLU (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLU (Cat# BD-PE5684)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.