BD-PE5677-EGFR (17W5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

EGFR (17W5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5677

产品名称

EGFR (17W5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

EGFR

推荐应用

反应种属

Human,Monkey,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human EGFR(NP_958440) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:500, IHC 1:100

预测分子量

42.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-EGFR mouse monoclonal antibody (BD-PE5677). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Western blot analysis of extracts (35ug) from 9 different cell lines using an anti-EGFR monoclonal antibody .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EGFR mouse monoclonal antibody. (BD-PE5677; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:250.

Immunohistochemical staining of paraffin-embedded human lung cancer with mouse anti-EGFR 1:200 dilution of 400ug/mL using HIER citrate pH6.0 pressure chamber. Expression in lung cancer cells are cytoplasmic and membraneous

Immunofluorescent staining of HeLa cells using anti-EGFR mouse monoclonal antibody (BD-PE5677, green, 1:50). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded human placenta with mouse anti-EGFR 1:200 dilution of 400ug/mL using HIER citrate pH6.0 pressure chamber. Placenta known to express high levels in the cytoplasm and membrane trophoblastic cells

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EGFR mouse monoclonal antibody. (BD-PE5677; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)