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HSPD1 (10H1) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE5673
产品名称
HSPD1 (10H1) Mouse Monoclonal antibody
类别
常规抗体
基因名称
Hsp60
推荐应用
WB
反应种属
Human,Mouse,Monkey,Dog,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human HSPD1 (NP_955472) produced in E.coli.
稀释度
WB 1:2000, IHC 1:150
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5673)

Western blot analysis of extracts (10ug) from 9 different cell lines by using anti-HSPD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:2000).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5673)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5673)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5673)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5673)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5673)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5673)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5673)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPD1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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