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NAPEPLD (NAPE-PLD) (2B4) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE5656
产品名称
NAPEPLD (NAPE-PLD) (2B4) Mouse Monoclonal antibody
类别
常规抗体
基因名称
NAPEPLD
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human (NP_945341) produced in HEK293T cell.
稀释度
WB 1:2000, IHC 1:150, FCM 1:100
预测分子量
45.4kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]). [supplied by OMIM]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5656)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5656)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5656)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NAPEPLD antibody (BD-PE5656), and then analyzed by flow cytometry.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAPEPLD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAPEPLD. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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