BD-PE5653-RAPGEF1 (13F11) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RAPGEF1 (13F11) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5653

产品名称

RAPGEF1 (13F11) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RAPGEF1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-RAPGEF1 mouse monoclonal antibody (BD-PE5653). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-RAPGEF1mouse monoclonal antibody. (BD-PE5653, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RAPGEF1 mouse monoclonal antibody. (BD-PE5653, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunofluorescent staining of HeLa cells using anti-RAPGEF1 mouse monoclonal antibody (BD-PE5653, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-RAPGEF 40 mouse monoclonal antibody. HIER ACCEL buffer (pH8.7) at 110C for 10 min, BD-PE5653 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-RAPGEF1 mouse monoclonal antibody. (BD-PE5653, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)