BD-PE5628-NME1 (17T2) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

NME1 (17T2) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5628

产品名称

NME1 (17T2) Mouse Monoclonal antibody

类别

常规抗体

基因名称

NME1

推荐应用

反应种属

Human,Rat,Monkey,Dog,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human NME1 (NP_937818) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100, IF 1:100

预测分子量

19.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NME1 mouse monoclonal antibody (BD-PE5628). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NME1 mouse monoclonal antibody. (BD-PE5628; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NME1 mouse monoclonal antibody. (BD-PE5628; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NME1 monoclonal antibody .

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-NME1 mouse monoclonal antibody. (BD-PE5628; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NME1 mouse monoclonal antibody. (BD-PE5628; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-NME1 mouse monoclonal antibody. (BD-PE5628; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NME1 mouse monoclonal antibody. (BD-PE5628; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NME1 mouse monoclonal antibody. (BD-PE5628; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunofluorescent staining of HeLa cells using NME1 mouse monoclonal antibody (BD-PE5628, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NME1 mouse monoclonal antibody. (BD-PE5628; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)