BD-PE5597-RLIM (13B8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RLIM (13B8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5597

产品名称

RLIM (13B8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RLIM

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human RLIM (NP_899196) produced in E.coli.

稀释度

WB 1:500~2000, IHC 1:500

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RLIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE5597) (1:500)

Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-RLIM monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RLIM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RLIM (Cat# BD-PE5597)(1:2000).