BD-PE5590-PLOD2 (12J13) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PLOD2 (12J13) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5590

产品名称

PLOD2 (12J13) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PLOD2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 359-640 of human PLOD2 (NP_891988) produced in E.coli.

稀释度

WB 1:2000

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PLOD2 mouse monoclonal antibody. (BD-PE5590) Dilution: 1:150

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PLOD2 mouse monoclonal antibody. (BD-PE5590) Dilution: 1:150

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLOD2 mouse monoclonal antibody. (BD-PE5590) Dilution: 1:150

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLOD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLOD2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.