BD-PE5586-ERG (2J5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ERG (2J5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5586

产品名称

ERG (2J5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ERG

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human ERG(NP_891548) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100

预测分子量

53.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERG mouse monoclonal antibody (BD-PE5586). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded mouse brain tissue using mouse anti-ERG mouse monoclonal antibody. HIER TEE buffer (pH9) at 110C for 10 min, BD-PE5586 (1:100). Detection was done with Klear Mouse (C/N ) DAB Chromogen Kit.

Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-ERG at 1:200 dilution of 0.8mg/mL and detection with Polink2 Broad HRP DAB. BD-PE5586 requires heat-induced epitope retrieval with ACCEL (pH8.7) biocare pressure cooker. The image shows prostate tumors staining in tumor cells.

Western blot of cell lysates (35ug) from 8 different cell lines (1: HeLa, 2: SV-T2, 3: A549. 4: COS7, 5: Jurkat, 6: MDCK, 7: PC-12, 8: MCF7).

Immunofluorescent staining of HeLa cells using anti-ERG mouse monoclonal antibody (BD-PE5586, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

IHC staining of paraffin-embedded carcinoma of human prostate using anti-ERG at 1:200 dilution of 0.8mg/mL and detection with Polink2 Broad HRP DAB. requires heat-induced epitope retrieval with EDTA (pH8.7) at 95-100C 20 min. The composit image shows 3 prostate tumors with no staining in tumor cells of figure A and B although strong nuclear staining is present in the endothelial cells. Figure C shows strong nuclear with weak cytoplasmic staining in prostate tumor cells.

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-ERG mouse monoclonal antibody. (BD-PE5586; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)