BD-PE5550-BTLA (9C17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BTLA (9C17) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5550

产品名称

BTLA (9C17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD272

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human BTLA(NP_861445) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150, IF 1:100

预测分子量

32.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTLA antibody (BD-PE5550), and then analyzed by flow cytometry (1:500).

Western blot analysis of extracts (35ug) from PBMCs by using anti-BTLA monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTLA. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Anti-BTLA mouse monoclonal antibody (BD-PE5550) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTLA .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BTLA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5550)