BD-PE5531-NCAM1/CD56 (9A8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

NCAM1/CD56 (9A8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5531

产品名称

NCAM1/CD56 (9A8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

NCAM1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 20-718 of human NCAM1 (NP_851996) produced in HEK293T cells.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100

预测分子量

94.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NCAM1 mouse monoclonal antibody (BD-PE5531). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD56 (NCAM1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE5531) (1:300).

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-NCAM1 mouse monoclonal antibody. (BD-PE5531; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-NCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE5531) (1:300)

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-NCAM1 mouse monoclonal antibody. (BD-PE5531; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)