BD-PE5514-ARL5B (6G3) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ARL5B (6G3) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5514

产品名称

ARL5B (6G3) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ARL5B

推荐应用

反应种属

Human,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human ARL5B(NP_848930) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

20.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

ARL5B (ARL8) belongs to a family of proteins that are structurally similar to ADP-ribosylation factors (ARFs; see MIM 103180). ARLs and ARFs are part of the RAS superfamily of regulatory GTPases. [supplied by OMIM, Nov 2010]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ARL5B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5514)

Anti-ARL5B mouse monoclonal antibody (BD-PE5514) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARL5B .

Flow cytometric Analysis of Jurkat cells, using anti-ARL5B antibody (BD-PE5514), (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARL5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARL5B(Cat# BD-PE5514). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.