BD-PE5504-ATG4B (1F8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ATG4B (1F8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5504

产品名称

ATG4B (1F8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ATG4B

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human ATG4B (NP_847896) produced in HEK293T Cell.

稀释度

WB 1:500~2000, IHC 1:2000

预测分子量

42.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE5504) (1:2000)

Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-ATG4B monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG4B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG4B (Cat# BD-PE5504)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.