BD-PE5500-DCX (15F1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

DCX (15F1) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5500

产品名称

DCX (15F1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

DCX

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human DCX (NP_835365) produced in HEK293T cell.

稀释度

WB 1:2000, IF 1:100, FCM 1:100

预测分子量

40.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ('double cortex' syndrome) in females and lissencephaly ('smooth brain' syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunofluorescent staining of 293T cells transiently transfected by pCMV6-ENTRY DCX using anti-DCX mouse monoclonal antibody (BD-PE5500, green, upper left; merged, upper right). Cell nuclei were stained with DAPI (blue, lower left). 293T cells transfected with empty vector plasmid served as negative control ( merged, lower right) (1:100).

Living HEK293T cells transfected with either plasmid (red) or empty vector (blue) were immunostained by anti-DCX antibody (BD-PE5500) or isotype control antibody (green), and then analyzed by flow cytometry (1:100).

Flow cytometric Analysis of Jurkat cells, using anti-DCX antibody (BD-PE5500), (Red), compared to a nonspecific negative control antibody , (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCX (Cat# BD-PE5500). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug) from 2 tissues by using anti-DCX monoclonal antibody (1:250).