Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HSD17B13 mouse monoclonal antibody, BD-PE5499 (1:200). *Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSD17B13 mouse monoclonal antibody, BD-PE5499 (1:200). *Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min.

Western blot analysis of overexpressed human HSD17B13 lysate (5ug , LEFT) and purified recombinant protein (0.02ug , RIGHT) by using anti-HSD17B13 antibody (BD-PE5499).

HEK293T cells were transfected with 10 different plasmids of human HSD cDNA ( HSD11B1, HSD17B2, HSD17B3, HSD17B4, HSD17B6, HSD17B7, HSD17B8, HSD17B10, HSD17B11, HSD17B13) for 48 hrs and lysed. Equivalent amounts of cell lysates (10ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B13 mouse monoclonal antibody. HSD17B13 was positive, while all others were negative. (BD-PE5499, 1：500)