HEK293T cells were transfected with 10 different plasmids of human HSD cDNA ( HSD11B1, HSD17B2, HSD17B3, HSD17B4, HSD17B6, HSD17B7, HSD17B8, HSD17B10, HSD17B11, HSD17B13) for 48 hrs and lysed. Equivalent amounts of cell lysates (10ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B13 rabbit monoclonal antibody. HSD17B13 and HSD17B11 were positive, while all others were negative(RM1392, 1：5000)

Western blot analysis of overexpressed human HSD17B13 lysate (5ug , LEFT) and purified recombinant protein (0.02ug , RIGHT) by using anti-HSD17B13 antibody (RM1392).