BD-PE5488-GOLM1 (15C8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GOLM1 (15C8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5488

产品名称

GOLM1 (15C8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

GOLM1

推荐应用

反应种属

Human,Monkey

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human GOLM1(NP_808800) produced in HEK293T cell.

稀释度

WB 1:200~500, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

45.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric Analysis of Hela cells, using anti-GOLM1 antibody (BD-PE5488), (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GOLM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GOLM1(Cat# BD-PE5488). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GOLM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5488)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GOLM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5488)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GOLM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5488)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GOLM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5488)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GOLM1 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GOLM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5488)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GOLM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5488)

Anti-GOLM1 mouse monoclonal antibody (BD-PE5488) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GOLM1 .