BD-PE5476-CD86 (16T18) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD86 (16T18) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5476

产品名称

CD86 (16T18) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD86

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CD86 (NP_787058) produced in HEK293T cell.

稀释度

WB 1:500~1000, FCM 1:100

预测分子量

38.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, May 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric analysis of living 293T cells transfected with CD86 overexpression plasmid , Red) using anti-CD86 antibody (BD-PE5476). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Flow cytometric analysis of living Raji cells, using anti-CD86 antibody (BD-PE5476, Red), compared to an isotype control (green) (1:100).

Flow cytometric analysis of living Ramos cells, using anti-CD86 antibody (BD-PE5476, Red), compared to an isotype control (green) (1:100).

Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CD86 monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD86 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD86 (1:1000).