BD-PE5378-PTK2 (FAK) (8R18) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PTK2 (FAK) (8R18) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5378

产品名称

PTK2 (FAK) (8R18) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PTK2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PTK2(NP_722560) produced in HEK293T cell.

稀释度

WB 1:200~2000, IHC 1:150, IF 1:100

预测分子量

119.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell by using anti-PTK2 monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5378)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5378)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5378)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5378)

Anti-PTK2 mouse monoclonal antibody (BD-PE5378) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTK2 .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5378)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTK2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.