BD-PE5372-FOXA2 (14I6) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

FOXA2 (14I6) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5372

产品名称

FOXA2 (14I6) Mouse Monoclonal antibody

类别

常规抗体

基因名称

FOXA2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2

稀释度

WB: 1:1000; IHC: 1:50; IF: 1:50-1:100; FCM 1:1,000

预测分子量

48.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-FOXA2 (BD-PE5372) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Western blot analysis of extracts (10ug) from 1 cell line by using anti-FOXA2 monoclonal antibody at 1:200 dilution.

HEK293T cells transfected with either pCMV6-ENTRY FOXA2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-FOXA2 mouse monoclonal (BD-PE5372, Dilution 1:1,000), and then analyzed by flow cytometry.

Immunofluorescent staining of HepG2 cells using anti-FOXA2 mouse monoclonal antibody (BD-PE5372) at 1:50 dilution.

Anti-FOXA2 mouse monoclonal antibody (BD-PE5372) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY FOXA2

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-FOXA2 monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA2 (Cat# BD-PE5372). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-FOXA2 (BD-PE5372) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).