BD-PE5343-PTK7 (CCK4) (6W2) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PTK7 (CCK4) (6W2) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5343

产品名称

PTK7 (CCK4) (6W2) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CCK4

推荐应用

反应种属

Human,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PTK7(NP_690619) produced in HEK293T cell.

稀释度

WB 1:200~500, IF 1:100, FCM 1:100

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene is a member of this subgroup of tyrosine kinases and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PTK7 antibody (BD-PE5343), and then analyzed by flow cytometry.

Anti-PTK7 mouse monoclonal antibody (BD-PE5343) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTK7 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTK7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTK7(Cat# BD-PE5343).