产品名称
SPY4 Rabbit Polyclonal Antibody
蛋白名称
Protein sprouty homolog 4 (Spry-4)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9C004
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9C004/entry
Mouse Swissprot No.
Q9WTP2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9WTP2
免疫原
Synthesized peptide derived from human protein . at AA range: 230-310
特异性
SPY4 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of a family of cysteine- and proline-rich proteins. The encoded protein is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. Activity of this protein impairs the formation of active GTP-RAS. Nucleotide variation in this gene has been associated with hypogonadotropic hypogonadism 17 with or without anosmia. Alternative splicing results in a multiple transcript variants. [provided by RefSeq, Jun 2014],
组织表达
Brain,Umbilical artery,
细胞定位
Cytoplasm . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor) (By similarity). Colocalizes with TESK1 in vesicular spots in the cytoplasm (PubMed:15584898). .
功能
domain:The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.,function:Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.,similarity:Belongs to the sprouty family.,similarity:Contains 1 SPR (sprouty) domain.,subunit:Interacts with the C-terminus of TESK1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
