BD-PE5215-MAP2K3 (MEK3) (9N10) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MAP2K3 (MEK3) (9N10) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5215

产品名称

MAP2K3 (MEK3) (9N10) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MAP2K3

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinan protein of human MAP2K3(NP_659731) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150, IF 1:100

预测分子量

39.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAP2K3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5215)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MAP2K3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5215)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5215)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAP2K3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5215)

Anti-MAP2K3 mouse monoclonal antibody (BD-PE5215) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K3 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K3. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.