BD-PE5160-BIRC7 (Livin) (11A19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BIRC7 (Livin) (11A19) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE5160

产品名称

BIRC7 (Livin) (11A19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

BIRC7

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human BIRC7 (NP_071444) produced in HEK293T cell.

稀释度

WB 1:1000, IHC 1:50, FCM 1:100

预测分子量

32.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide.

期货

2周

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric analysis of Hela cells, using anti-BIRC7 antibody (BD-PE5160), (Red) compared to a nonspecific negative control antibody (Blue).

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BIRC7mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5160, Dilution 1:50)

Flow cytometric analysis of Jurkat cells, using anti-BIRC7 antibody (BD-PE5160), (Red) compared to a nonspecific negative control antibody (Blue).

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BIRC7 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-BIRC7 monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC7(Cat# BD-PE5160). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.