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MAPK1 (ERK2) (12S3) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE5143
产品名称
MAPK1 (ERK2) (12S3) Mouse Monoclonal antibody
类别
常规抗体
基因名称
MAPK1
推荐应用
WB
反应种属
Human,Dog,Rat,Monkey,Mouse
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.
稀释度
WB 1:2000, IF 1:100, IP 2-4ug/mg
预测分子量
41.4kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2a
背景介绍
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.

Anti-MAPK1 mouse monoclonal antibody (BD-PE5143) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK1 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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