BD-PE5140-MAPK1 (ERK2) (18I14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MAPK1 (ERK2) (18I14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5140

产品名称

MAPK1 (ERK2) (18I14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MAPK1

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.

稀释度

WB 1:1000~2000, IHC 1:50, FCM 1:100, IP 2ug/500ul

预测分子量

41.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK1 antibody (BD-PE5140), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5140)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5140)

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-MAPK1 monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1 (Cat# BD-PE5140). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.