BD-PE5116-BCL2L1 (11R12) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BCL2L1 (11R12) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE5116

产品名称

BCL2L1 (11R12) Mouse Monoclonal antibody

类别

常规抗体

基因名称

BCL2L1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human BCL2L1(NP_612815) produced in HEK293T cell.

稀释度

WB 1:1000, IHC 1:150

预测分子量

25.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BCL2L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5116) (1:150)

Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-BCL2L1 monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-BCL2L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5116) (1:150)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BCL2L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5116) (1:150)

Western blot analysis of extracts (35ug) from 3 tissue lysates by using anti-BCL2L1 monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCL2L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE5116) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2L1 (1:500).

Western blot analysis of extracts (35ug) from 11 cell lines lysates by using anti-BCL2L1 monoclonal antibody (1:500).