BD-PE5115-Bcl-XL (6X17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Bcl-XL (6X17) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5115

产品名称

Bcl-XL (6X17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

BCL2L1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Recombinant fragment expressed in E.coli corresponding to amino acids 1-212 of human Bcl-XL

稀释度

WB: 1:200 - 1:1000, IF (1:100)

预测分子量

25.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

Bcl-XL belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCL-XL mouse monoclonal antibody. (BD-PE5115) (1:150)

Immunofluorescent staining of HT29 cells using anti-BCL2L1 mouse monoclonal antibody (BD-PE5115) (1:50).

Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-BCL-XL monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BCL-XL mouse monoclonal antibody. (BD-PE5115) (1:150)

Anti-BCL2L1 mouse monoclonal antibody (BD-PE5115) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCL2L1 (1:100).

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-BCL-XL monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2L1 (Cat# BD-PE5115).

Western blot analysis of extracts (35ug) from 11 cell lines lysates by using anti-BCL2L1 monoclonal antibody (1:500).