产品名称
AMD Rabbit Polyclonal Antibody
蛋白名称
Peptidyl-glycine alpha-amidating monooxygenase (PAM) [Includes: Peptidylglycine alpha-hydroxylating monooxygenase (PHM) (EC 1.14.17.3); Peptidyl-alpha-hydroxyglycine alpha-amidating lyase (EC 4.3.2.5) (Peptidylamidoglycolate lyase) (PAL)]
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P19021
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P19021/entry
Mouse Swissprot No.
P97467
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P97467
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P14925
免疫原
Synthesized peptide derived from human protein . at AA range: 380-460
特异性
AMD Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016],
组织表达
Epithelium,Heart,Kidney,Lung,Thalamus,Thyroid carcinoma,
细胞定位
Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein . Secretory granules. .; [Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted. Secreted from secretory granules.; [Isoform 4]: Secreted. Secreted from secretory granules.
功能
alternative products:Additional isoforms seem to exist,catalytic activity:Peptidylamidoglycolate = peptidyl amide + glyoxylate.,catalytic activity:Peptidylglycine + ascorbate + O(2) = peptidyl(2-hydroxyglycine) + dehydroascorbate + H(2)O.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,cofactor:Binds 2 copper ions per subunit; For the monoxygenase reaction.,cofactor:Zinc; for the lyase reaction.,enzyme regulation:Inhibited by EDTA, phenylglyoxal and diethyl pyrocarbonate.,function:Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.,similarity:Contains 5 NHL repeats.,similarity:In the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.,similarity:In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.,subcellular location:Secreted from secretory granules.,subunit:Monomer. Interacts with RASSF9.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
