BD-PE5074-RAD51L1 (10V16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RAD51L1 (10V16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5074

产品名称

RAD51L1 (10V16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

Rad51L1

推荐应用

反应种属

Human,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 119-346 of human RAD51L1(NP_598193) produced in E.coli.

稀释度

WB 1:500, IF 1:100, FCM 1:100

预测分子量

42kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-RAD51L1 monoclonal antibody at 1:200 dilution.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAD51L1 antibody (BD-PE5074), and then analyzed by flow cytometry.

Anti-RAD51L1 mouse monoclonal antibody (BD-PE5074) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RAD51L1 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD51L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD51L1(Cat# BD-PE5074). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.