BD-PE5057-BMP4 (12N17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BMP4 (12N17) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5057

产品名称

BMP4 (12N17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

BMP4

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Recombinant fragment expressed in E.coli corresponding to amino acids 293-408 of human BMP4

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:500, IHC 1:100

预测分子量

46.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-BMP4 mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-BMP4 very specifically recognizes BMP4 antigen on OriGene protein microarray chip.

Immunohistochemical staining of paraffin-embedded human placenta using BMP4 , mouse monoclonal antibody at 1:100 dilution of1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE5057 requires heat-induced epitope retrieval with Accel pH8.7 at 110°C for 3min using pressure chamber/cooker. The image shows strong membranous and cytoplasmic staining.

HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY BMP4 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMP4. (Cat# BD-PE5057)

Immunohistochemical staining of paraffin-embedded human small intestine using BMP4 , mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE5057 requires heat-induced epitope retrieval with Accel pH8.7 at 110°C for 3min using pressure chamber/cooker. The image shows strong membranous and cytoplasmic staining.

Immunofluorescent staining of HeLa cells using anti-BMP4 mouse monoclonal antibody (BD-PE5057, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded human stomach carcinoma using BMP4 , mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE5057 requires heat-induced epitope retrieval with Accel pH8.7 at 110°C for 3min using pressure chamber/cooker. The image shows strong membranous and cytoplasmic staining.

Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-BMP4 monoclonal antibody