BD-PE5048-DPP8 (1H16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

DPP8 (1H16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE5048

产品名称

DPP8 (1H16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

DPP8

推荐应用

反应种属

Human,Dog,Rat,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human DPP8(NP_569118) produced in HEK293T cell.

稀释度

WB 1:500~1000, IHC 1:150

预测分子量

101.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DPP8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE5048)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DPP8 monoclonal antibody.

Figure from citation: Western blot analysis of protein levels of DPP4/8/9 in 293E cells transfected with pcDNA3.1 (Mock), pcDNA3.1-hDPP8 (DPP8) and pcDNA3.1-hDPP9 (DPP9), the purified recombinant human DPP4 protein (rhDPP4) was used as a positive control for the DPP4 antibody, antibodies targeting human DPP4/8/9 and β-actin were purchased from OriGene.

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-DPP8 monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPP8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP8(Cat# BD-PE5048). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.