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SEPT1 (3I8) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE4984
产品名称
SEPT1 (3I8) Mouse Monoclonal antibody
类别
常规抗体
基因名称
SEPTIN1
推荐应用
WB
反应种属
Human
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human SEPT1(NP_443070) produced in HEK293T cell.
稀释度
WB 1:200~1000
预测分子量
41.8kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2a
背景介绍
This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SEPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEPT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEPT1 (Cat# BD-PE4984). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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