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WDR4 (12W4) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE4983
产品名称
WDR4 (12W4) Mouse Monoclonal antibody
类别
常规抗体
基因名称
WDR4
推荐应用
WB
反应种属
Human
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human WDR4(NP_387510) produced in HEK293T cell.
稀释度
WB 1:2000, IF 1:100, FCM 1:100
预测分子量
45.3kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-WDR4 antibody (BD-PE4983), and then analyzed by flow cytometry.

Anti-WDR4 mouse monoclonal antibody (BD-PE4983) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY WDR4 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR4(Cat# BD-PE4983). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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