BD-PE4976-FBXW7 (6U16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

FBXW7 (6U16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4976

产品名称

FBXW7 (6U16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

FBXW7

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 1-354 of human FBXW7 (NP_361014) produced in E.coli.

稀释度

WB 1:2000, IHC 1:150

预测分子量

79.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FBXW7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4976)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-FBXW7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4976)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-FBXW7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4976)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FBXW7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4976)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FBXW7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4976)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FBXW7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4976)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXW7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXW7. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.